Biocatalytic Oxidation of Primary & Secondary alcohols by using Candida Parapsilosis ATCC 7330

Abstract

Abstract Oxidation of α-hydroxy esters and aromatic alcohols to the corresponding carbonyl compounds is an important and challenging transformation both at laboratory and industrial scales. It belongs to the most fundamental processes in organic chemistry. Recent efforts are dedicated to green catalytic oxidation methods. Conventionally, oxidation is brought about by utilizing stochiometric /superstochiometric amounts of inorganic oxidants like Ru,Rh and Ir or Oppenauer oxidation (with Al,Zr and lanthanides) which are incongruous to the principle of green chemistry. In the present study, optimisation was done for non-enantioselective biocatalytic oxidation of α-hydroxy ester (racemic ethyl 2-hydroxy ,4- phenylbut-3-enoate) and aromatic alcohol (3-phenyl prop-2-enol (cinnamyl alcohol) ). The oxidation was achived by using whole cells of Candida parapsilosis ATCC 7330 and the reaction conditions were optimized to get 61% oxidised product within 5 mins at 25°C.